Minggu, 19 Oktober 2014

Tugas Teknik Laboratorium


TASK TECHNIQUE OF LABORATORY
“The Journal Technique of Laboratory “


By :
Nama    : Bella Rhea lavifa Sanjaya
NIM       : 120210153038



STUDY PROGRAM OF BIOLOGY EDUCATION
DEPARTEMENT OF MATHEMATIC AND SCIENCE
FACULTY OF TEACHER TRAINING AND EDUCATION
UNIVERSITY OF JEMBER
2012



The Journal Technique of Laboratory
This is the first time I knew laboratory techniques. It felt like a little tricky and confusing if I have to set anything that should be done in the mastery of laboratory techniques. A lot of the equipment I need to understand and policies should I understand about the lab. It requires maturity in attitude. But since the beginning, I hope I can get through it well and able to deal with all the obstacles I would encounter while studying technique of laboratory
After several meetings took place, lecturer Laboratory Techniques replaced by Mrs Kamalia Fikri, S. Pd., M. Pd. My first impression, she is a friendly, industrious and sociable. Although she was very busy, but she still can run the system with good laboratory techniques course, is to run a course via the website. So even if not face to face, the discussion can still run, the task can still be collected on time and he was also able to monitor who is active students. So that lectures via highly efficient website.
At that time I came very late. With vantofel shoes made ​​uncomfortable, I walked quickly from boarding. Arriving in class X1, I saw Mrs. Kamalia Fikri, S. Pd., M. Pd has been dealing with laptop sitting in front of my friends. At that time, I along with my late wulan also soon find a seat. Sadly shortly after we sat down, the lesson is over and we were confused with what the task given to us.
"Microtome" I heard it first from my friend who happened to be recorded Rasmiyana task for me. I was more confused after I asked she how the writing of the microtome. The first thing I did for finding out what it is about microtome microtome, I still do not understand what it microtome. Hehehe .. feels confused. But I started to source for resources and eventually i finish the required task. A paper containing the definitions, functions, method of storage and how to get it. Since the task has been completed, I was sent to the address of the task http://kamalia.fkip.unej.org/ website.
A microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a tool used to cut extremely thin slices of material, known as sections. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light orelectron radiation. Microtomes use steel, glass, or diamond blades depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare sections of animal or plant tissues for light microscopy histology. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial grade diamond knives are used to slice hard materials such as bone, teeth and plant matter for both light microscopy and for electron microscopy. Gem quality diamond knives are used for slicing thin sections for electron microscopy.
The Function of Microtome
Microtomy is a method for the preparation of thin sections for materials such as bones, minerals and teeth, and an alternative to electropolishing and ion milling. Microtome sections can be made thin enough to section a human hair across its breadth, with section thickness between 50 nm and 100 µm.

Treatment Microtome
Microtome should be covered with plastic, or put back in the box when not in use. Do not move microtome by holding the moving parts, as they may interfere with the accuracy. Before and after use, preferably paraffin microtome cleared of debris by wiping with a cloth that has been dampened with xilol. Microtome must be oiled to prevent wear and congestion.
Why should preferably paraffin microtome cleared of debris by wiping with a cloth that has been dampened with xilol?
As i know it caused the function of xilol that used for cleaning paraffin microtomes of debris can damage the coating of fungus on the lens. so expect microtome maintained properly. besides the advantages of xilol is a commonly used, inexpensive, and works fast
A week after the task was completed, a new task was following the discussion onlinne. Many of my friends who are still confused as to what the online discussions, so anything with me who are also confused that how to begin the online discussions. There are some tools used for discussion in the course of this laboratory technique, here me and my friends ask questions, answer, respond so the discussion continues to run fluently. After discussing it, I know more detail about the tools and i can make a conclusion about the tools that be a topic of discussion as follows:
1. ULTRASONIC CLEANER UNIT  


   1. Description
Based on the topics that I downloaded, Ultrasonic Cleaner Unit is a tool to clean by using ultrasonic waves so that we need not rub or scrape dirt stick to the goods that are difficult to clean. 
   2. The Function
The function is to suitable for cleaning like Glasses, jewelry, dentistry equipment, printer head, comb, tattoo equipment, dentures, watches, and so forth.
   3. How to use it
How to use it very easy, living water intake approximately 60% of the capacities of water into the container, and put the solution cleaners (cleaning solution depending on what tools to be cleaned) and then turn the key ON.
   4. How to care it
After use it dont forget to turn off the tools. Then it should be stored at room temperature and do not put the damp. This tool can also be stored in a special cupboard. To clean this material just clean with dry cloth and make sure that the machine in off condition.
For the power source, It does need electricity, as "An Ultrasonic generator energizes the transducers. The generator transforms the electrical energy from the power source into a suitable form for efficiently energizing the transducers at the desired frequencies. The generator produces a electronic signal of high voltage and sends it to the transducers. When the transducers receive the signal they will respond by changing shape as long as the signal is applied. The response range of the transducer determines the frequency of the generator. Since the response range of the transducer is narrow the signal from the generator must be close to the response range of the transducer", so  the power source is depend on the type of ultrasonic claner it self. but some source  telling battery as their source because all of them discuss about portable ultrasonic cleaner.
From that i asked “Is there negative effect of ultrasonic cleaner unit?”, but no one answered it.
2. DESICCATOR OR EKSIKATOR
1. Description
Desiccator is another name of eksikator. That is a device made of glass shaped two-tiered pot filled bottom drying agents such as silica gel so that the effect of water vapor during drying can be absorbed by the silica gel. Because it is made of thick glass, it is classified Desiccator laboratory weighing equipment. Mainly because of the hard cover is removed in the cold because vaseline coated.
Various Desiccator or eksikator
Desiccator / eksikator there are two kinds, namely:
1.  Ordinary Desiccators
2. Vacuum desiccator (Vacuum)
So what is different between Ordinary Desiccators and Vacuum desiccator (Vacuum)???
1.  Ordinary Desiccators
Ordinary desiccators is to maintain low humidity at a pressure less than 20 mmHg or the other pressures specified in the monograph. does not have a valve that can open the lid, so it can not connect the hose to the pump.
2. Vacuum desiccator (Vacuum)
Vacuum desiccator on the lid there is a valve that can open the lid, which is connected by a hose to the pump.
   2. The Function
      • The storage of samples should be free of water
      • Drying and cooling the sample that will be used to test the moisture   content
3. How to use it
1.        How to open a closed desiccator is by sliding sideways.
2.        Put new sample out of the oven or to be dried and cooled
3.        Then put the cap back in the same way, namely by opening earlier in the sliding laterally.
4.        Notice the silica gel that serves as a water absorbent money. Silica gel absorbs moisture could still blue, if silica gel has changed to pink it needs to be heated in the oven at 105 ° C until the blue color again. The form of pink color is caused by chemical fillers or material will desiccator dried or extracted
4. How to care it
 After using desiccators, desiccator should be kept private, so desiccator safe and protected from damage. otherwise it uses dseikator should follow the instructions properly so durable desiccator.


3. LABORATORY INCUBATOR (CULTURE)
1. Description
Incubator is a place to keep something in several temperatures. There are some types of incubator, just like egg incubator, microbiology incubator, and baby incubator.
As i know from the disscussion, egg incubator: This often results in higher hatch rates due to the ability to control both temperature and humidity baby incubator: A neonatal incubator is a device consisting of a rigid box-like enclosure in which an infant may be kept in a controlled environment for medical care microbiology incubator: is a device used to grow and maintain of course microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.
2. The Function
In biology, an incubator is a device used to grow and maintain of course microbiological cultures or cell cultures. Incubators are also used in the poultry industry to act as a substitute for hens.
3. How to use it
Set a temperature on the thermostat and when the temperature dips below this point, a heating element comes on(electrical element is common). One the temperature reaches another point, the heating element is turned off. It keeps whatever is in it warm and alive.
4. How to care it
Keep incubator in a dry place and don’t get sunlight directly. Keep the door close and don’t open the door too often to keep the temperature inside.
4. DIAL O GRAM BALANCE
1. Description
To measure the mass that is necessary to use a measuring device called a dial 0 gram balance. The less sensitive of the two balances you will be using is called the Dial-o-gram balance. It can weigh objects and samples (up to 300 grams) to a precision of 1/100th of a gram--that is, to the second decimal place (0.01).
Specification of Tools
Ohaus 310-00 Dial-O-Gram Balance.High precision accuracy, no leveling required. 310 gram capacity x 0.01 gram resolution
Specification
Power
None
Main Color
White
Display
Dial & beams
Tare Feature
Yes
Weighs With
Suspended pan
Platform Size
0.5" deep x 3.5" diameter
Scale Size
18" x 12" x 9"
Weighing Modes
1
Then i asked, “is it possible to dial o gram balance showed less precise measurements that we do?” and the answer is Errors include: 1. weighing error when first measured but not scala gauge at zero 2. the calculation results when adding the number of arms balance and dial 3. observer error when viewing scala gauge 4. Objects measured <0.01 grams, so the accuracy is less accurate 5. presence of external factors such as damage to equipment and other.
The Weakness Of DIAL O GRAM BALANCE
If asked shortage of dial-o-gram balance I can not say the specific, because when compared to the measurement tool I have ever used in a physics lab (Ohaus balance), better tools and carefully, perhaps by using this tool, determine the error in the measurement results more easily and accurately. But it is undeniable that this tool has shortcomings: 1. They are more expensive 2. Treatment more difficult 3. penggunkannya rather complicated way, because it combines the working principle Ohaus balance and micrometer screw 4. Precision is only 0.01 grams 5. measurement results are still a number that should count himself, not digital numbers straight out 6. etc maybe this is all I can say, if you are dissatisfied
2. The Function
The tool have function to measure mass of object with minute 0,01 gram.
3. How to care it
- Before you use the tool, you must know the condition of tool
- You must use the tools carefully
- After you use the tool, you should back the condition like a first
- Store the tool in room store, room store must in good condition
   you must arrange the humidity, temperature and light
5. INSECT NET
1. Description
Insect nets are made from a lightweight and strong material, which is muslin and calico. Long stalks nets about 75-100 cm long and 50 cm nets. Mouth open net with a diameter of about 30 cm. Circle frame made of wire mesh mouth hard and strong. The length of muslin bags about two times the length of the net mouth diameter circle. Insect nets can be used in two ways, namely Swung at the plant and Ran around planting.
Insect net include in laboratory tools because insect nets can be used to catch insects. Insects that can be used as an object of observation in biology so that the tool can be inserted in a particular laboratory biology. To use the lab equipment you could ask for help in laboratory workers.
2. the Function
Insect net is an invaluable tool for capturing flying insects are active and the tool is used with the help of hands to mengkap active flying insects, such as butterflies, dragonflies, bees, etc..
3. How to care it
For treatment of insect net is not difficult, among others:
a. Using insect nets as needed. That is to catch insects in the air to use the net active air as well as water nets and nets swinging.
b. After use, insect nets are stored in a safe place and prevented from reach of children.
6. RESPIROMETER
1. Description
Respirometers are designed to measure respiration either on the level of a whole animal (plant) or on the cellular level. These fields are covered by whole animal and cellular (or mitochondrial) respirometry, respectively.
2. The Function
A respirometer is a device used to measure the rate of respiration of a living organism by measuring its rate of exchange of oxygen and/or carbon dioxide. They allow investigation into how factors such as age, chemicals or the effect of light affect the rate of respiration.
As i know A respirometer is a device which is used to measure respiration, where organisms exchange carbon dioxide and oxygen as part of their metabolism. These devices can be used to study plants and animals alike to learn more about their respiratory patterns, and to monitor the health of an individual organism, using known information about that organism as a baseline. Respirometers are sold by a number of scientific supply companies, and they are also improvised in lab environments, since the mechanics are relatively straightforward.
3. How to use it
A simple whole animal respirometer designed to measure oxygen uptake or CO2 release consists of a sealed container with the living specimen together with a substance to absorb the carbon dioxide given off during respiration, such as soda lime pellets or cotton wads soaked with potassium hydroxide. The oxygen uptake is detected by displacement of manometric fluid in a thin glass U-tube connected to the container. When the organism takes in oxygen it gives off an equal volume of carbon dioxide. As this is absorbed by the soda lime, air is sucked in from the U-tube to keep the pressure constant, displacing the liquid. The rate of change gives a direct and reasonably accurate reading for the organism's rate of respiration.
4. How to care it
As changes in temperature or pressure can also affect the displacement of the manometric fluid, a second respirometer identical to the first except with a dead specimen (or something with the same mass as the specimen in place of the organism) is sometimes set up. Subtracting the displacement of the second respirometer from the first allows for control of these factors.
7. SOIL TESTER
1. Description
A soil test is the analysis of a soil sample to determine nutrient and contaminant content, composition and other characteristics, such as acidity or pH level.Soil testing is often performed by commercial labs that offer a variety of tests, targeting groups of compounds and minerals. There may be some advantage to using a local lab that is familiar with the chemistry of the soil in the area where the sample was taken. This enables technicians to recommend the tests most likely to reveal useful information.
Then i asked “,why does usually the best time of soil tests can be done when spring? and how if we do it when in another season?” and the answer is The purpose of soil testing program in the spring is to make farmers aware of fertilizer use efficiency. where the suitability of fertilizer can help to get the desired level. Test the soil brought increased agriculture production. Soil tests done to determine the amount and type of fertilizer are needed for different types of plants that vary from field to field and even on the same land. Soil tests helps to ensure maximum profits by providing the right amount of fertilizer at the stand. Without a soil test is difficult for farmers to apply the right kind of fertilizer. Using the wrong fertilizer reduces crop yields. Soil testing is usually done to find out its nutrients. besides, taah testing was also conducted during the fall (after harvest), it aims to determine the condition of the soil fertility so that we can adjust fertilizer for the next planting season. So i think soil tester can also use for another season.
2. The Function
1. This function for measurement Ph and soil hygro with its units
2. Tests are usually performed to measure the expected growth potential of a soil.
3. A soil test measures fertility, indicates deficiencies that need to be remedied, and
4. Soil testing is used to facilitate fertilizer composition and dosage selection for land employed in both agricultural and horticultural industries.
5. Determines potential toxicities from excessive fertility and inhibitions from the presence of non-essential trace minerals.
6. The test is used to mimic the function of roots to assimilate minerals. The expected rate of growth is modeled by the Law of the Maximum.
3. How to use it
1. put on the tip of tool into the soil until their cell site in the soil and wait for minutes letter
2. looking the scale for detect the power hydrogen (pH)
3. Pressing the bottom beside side the tool for determining the hygro solid after waiting for a few minutes
4. lookingthe smooth scale under of it as indicator the hygro soil
4. How to care it
-Don’t take in the higro condition (lembab) because can corrosion.
-After use it, we must clean it with sterril cotton
-After use it we must also  entering it to the its box
8. PHOTOMETER
1. Description
Photometer consists of the following sections:
1.        Monitor, serves to show how much light intensity is measured.
2.        Detectors, used to detect or determine the intensity of the light.
3.        Cable, used to connect the detectors with Photometer.
4.        Button controls, used to turn on and off Photometer, and to determine the intensity of the use.
Based on my asked “Is there any other types of Photometer?”, i know that No, actually the name Photometer not only used by tools that I described. There is another one called Photometer instrument is a device used to measure and adjust the light on the camera.
2. The Function
Photometer is an instrument used to measure the intensity of light or radiation. Or it could be said that "Photometer" is a common tool used to measure how much light intensity.
3. How to use it
   1. Turn on the photometer by turning the control knob.
   2. Set the size of the intensity of the use.
   3. Place the detector on the light to be measured.
   4.On the monitor will show how much the intensity of the light  produced.
  5. When finished turn off the photometer and store in a safe place.
4. How to care it
After use, should be turned off to save power Photometer Photometer. Photometer then put in a safe place to avoid damage..
9. MICROSCOPE
1. Description
In this introductory biology course, you will be using two type of microscope. The compound microscope and the dissecting microscope, it is imperative that you learn how to handle and care for they are expensive tools. It is also important that you develop good microscope technique because you will be asked to take critical micros couple examinations throughout this course. You will certainly conserve a great deal of time and observe much more of the fine detail of spearmen if you became proficient. Now in the use of the microscope (Robert ; 1993).

Microscope ( Latin ; micro : small, scopium : vision), that function is increase separation ability until possible to observe object which are very smooth.
2. The Function
The microscope is a tool used to see objects that are very small that can’t be observed with the naked eye
3. How to use it
1. Place the microscope in the light , open the diaphragm until maximum;
2. Arrange the position of the flat/concave mirror so that the condenser glass became light;
3. Increase the condenser until maximum by rotating the condenser button;
4. Place the prepared on the stage of microscope;
5. Pull down the tube microscope until objective lens nearly touching the cover glass;
6. Look through the ocular lens, a prepared until focus by turning regulator coarse and smooth control.
Note : when using microscope, using magnification of ocular lens and objective lens from weak to coarse. Arrange slit diaphragm until adequate lighting.
4. How to care it
1. Do not direct the reflector to the light source; light exposure can injure your eyes.
2. Start with using the weakest objective lens magnification in each observation.
3. Make sure that the slide is covered by the glass cover.
4. Adjust the stage level so that stage is in a level position.
5. Do not touch the lens with your fingers, the dust from your fingers can stimulate fungus growth on the lens.
6. Avoid direct contact between the objective lens and the slide object because it can damage the lens.
7. Clean the lens with cloth or lens tissue.
8. Do not keep the microscope in a humid place. If you place it in a cabinet. Place a lamp to avoid humidity. If necessary, keep the microscope in a container with silica gel.
9. Carry the microscope with two hands. Keep one hand on the microscope base and the other underneath the arm.
Price : Rp 2.500.000,00
If there is no price difference between the type of microscope that one type to another?? Actually of course not because the actual price of the microscope in accordance with its type.
- PAYMENT CAN COD (Pay At Home or Office)
- FREE INTER REGIONAL JABODETABEK
Please call / SMS to 021-37289110 / 021-97552359 / 08561761134 / ihzans@gmail.com
Facebook: Sanes Medical
10. CONDUCTIVITY METER
1. Description
Conductivity meter has the following sections.
1. Monitor, used to tell how big the conductivity of an object.
2. On / Off button is used to turn on and off conductivity meter.
3. Detectors / Testimeter, serves to detect konductivitas a material is measured.
4. Connecting cables, used to connect the meter with a conductivity detector.
2. The Function
Conductivity meter is a device used to measure the conductivity or the conductivity of an object. This tool has a monitor that is used to determine the conductivity of an object or substance and is equipped with testimeter (a tool to test / detect).
3. How to use it
How to use this tool are as follows.
1. Place objects or substances to be measured conductivity.
2. Turn the ON button on the conductivity meter and insert or install detectors on objects / substances were observed.
3. On the monitor conductivity meter will show how much the conductivity of an object or substance.
4. Press the Off button to turn off the conductivity meter.
5. Clean the conductivity meter after use and store in a dry place
4. How to care it
    1. put on dry place, dont put on wet place.
Why shouldn’t we put on wet place? Because if conductivity meter placed in the wet will be easily damaged. So it should be placed in a dry place, in order to remain durable conductivity meter and can be used in a long time.
    2. clean the dirt on the tool.
    3. after use the tool, place on cupboard.
11. HYGROMETERS
1. Description
Hygrometer is derived from the Greek meaning hugros moist and metreo mean measure. Hygrometers is a kind of tool to measure the level of moisture in a given place. Hygrometers Typically this tool is placed in storage for items that require humidity stage awake as safe camera. Lower moisture will prevent the growth of mold is an enemy on the equipment.
Who first discovered the Hygrometer?
Based on the literatur the first is guillaume amontons but in literatur is many difference argument about the first discovered hygromater.
So what is on his mind when creating a hygrometer??? I still dont know it till right now.
2. The Function
Hygrometers serves to measure the level of moisture in a given place

3. How to use it
By putting in place the measured humidity. Then wait and read the scale, the scale of moisture is usually marked with the letter "h" or if the temperature by degrees celcius.
4. How to care it
After use hygrometers should be cleaned before storing to prolong resistance hygrometers. Save hygrometers in place that is not too dry and not too moist.
12. THERMOMETERS
  
 1. Description
A thermometer usually consist of a containing hollow glass pipe of hydrogen mercury or alcohol, and dilution tabletop is air vacuum. Thermometer made pursuant to principle that hydrogen volume will change if heated or made cool. Hydrogen volume will increase if heated, while if made cool by hydrogen volume will decrease. Go up or go down the hydrogen him used as by reference to determine temperature a[n object. Hydrogen upon which filler of thermometer there is two kinds of, that is alcohol and mercury. So, in the reality the hydrogen have some advantage and disadvantage.
2. The Function
Thermometer is Appliance used to measure object temperature correctly and expressing it with thermometer angka.

3. How to use it
a. Mercury Thermometers
1. Prior to the change in temperature, the volume of mercury is on the initial conditions.
2. Changes in the ambient temperature around the thermometer mercury response to changes in volume.
3. The volume of mercury will expand if the temperature increases and will shrink when the temperature decreases.
4. The scale on the thermometer will indicate the temperature value corresponding circumstances.
b. Alcohol Thermometers
1. Prior to the change in temperature, the volume of alcohol is on the initial conditions.
2. Changes in the ambient temperature around the thermometer responds to changes in the volume of alcohol.
3. The volume of alcohol will expand if the temperature increases and will shrink when the temperature decreases.
4. The scale on the thermometer will indicate the temperature value corresponding circumstances.
c. Clinical Thermometers
Leaving the tip of the thermometer under the tongue, put in the back of the mouth or under the arm. The mouth is closed, allow up to 1 minute to measure body temperature. Remove the thermometer and read the thermometer indicator. Under the arm (for children under 3 years old)

4. how to care it
Each finish is used to measure the temperature of an object, you should use the thermometer in the clear lap, to keep the temperature stable. Then keep the thermometer in place with a temperature standard, meaning there should not be too cold and too hot.
Mercury includes hazardous substances (often used "hard water") so that the mercury thermometer dangerous if the tube broke. Actually what danger would occur if the mercury touched one of of our body? The skin will blister and red and this is very dangerous, so its use should be careful. And how if it occurs to us? if touch bit of skin surface we can handle it by yourself with iodine and antiseptic and cover with a bandage. but if it's serious we immediately took him to the emergency room.
13. BAROMETER
1. Description
Barometer , Term ' barometer' introduced  at 1665-1666 by a natural man of science of so called Irlandia of Robert Boyle. The Word alighted from Greek term of báros meaning ' weight, wight' and metron meaning ' size measure', meaning air heavy classification.
2. The Function
To know or measure air pressure.A barometer is a scientific instrument used in meteorology to measure atmospheric pressure. Pressure tendency can forecast short term changes in the weather. Numerous measurements of air pressure are used within surface weather analysis to help find surface troughs, high pressure systems, and frontal boundaries.
3. How to use it
1. Putting down at place to be measured by its air pressure
 2. Note scale which is ditunjukan by barometer
 3. Scale which is show by barometer represent air pressure storey level
     at area
4. How to care it
After using this appliance , then cleared of  dust or dung ,then reentered in depository cupboard
14. SECTION TOOLS / EQUIPMENT SURGERY
1. Description
Surgical tool is a tool designed to be used for surgical activities, such as dissecting animals, humans, and so on. Some sections are required in the manufacture of botanical preparations
2. The Function
Surgical instrument consisting of a leather wallet that contains the following tools:
1. Tweezers (the pointy end), is used to retrieve or pulling the organs of the animals were sacrificed, organs separate from one another.
2. Scalpel. Leaf blades mounted on a shaft surgical scalpel and used for skinning animals are dissected, cut off body parts and so on. Leaf blades and stems knife is one unit. The blades are of two kinds, namely the pointy toes and that led to no taper. This model can be sharpened, while the generally thrown off course when it is blunt.
3. Surgical Scissors (straight), is used to cut the parts of organs to be observed, such as the intestines, heart, blood vessels, and so on. Commonly used to hold the first opening on the body part to be examined.
4.  Board surgery is used as a surgical
5. stemmed needle (the tip bent, blunt), is used to remove the organs located at the bottom, to trace the vein or artery to prevent damage.
Two kinds of blade, namely the pointy toes and that led to no taper. Then, what are the difference use of the pointy toes and that led to no taper ? In the pointy toes for skinning animals are dissected, cut off body parts and so on. And that led to no taper for to observe organs from these animals.
3. How to use it
Tweezers:           1. Holding the center
                       2. Pinning objects to be studied
Scalpel:              1. Holding his part
                       2. Using a knife to dissect / unlock objects
Scissors:             1. Holding his part
                      2. Loosen the grip and then use scissors to dissect the object
Surgical board:    1. Setting up the board
                       2. Placing objects on board surgery
Stemmed needle: 1. Objects on a board stretched surgery
                       2.Giving stemmed needle on the end - the end of a   stretched object
4 How to care it
Having used the tool section of washed until clean, then wipe using a soft cloth to maintain the shape and sharpness of the tools section. Save on air conditioning to be more safe and secure condition.
15. PLANKTON NET
1. Description
Plankton net is a net with a mesh size adapted to plankton. The use of plankton nets in addition to practical samples also obtained quite a lot. Ordinary net plankton nets made ​​of nylon, usually conical in various sizes, but the average leng this 4-5 times the diameter of the net mouth.The net serves to filter the water and plakton the rein.
Must we sewed the nylon of plankton net is cone? What is the function of that shape?. As i know, I think it's easier to filter the water and plakton therein. Therefore caught plankton depends on the size of the mesh size, the size of the mesh size used should be tailored to the type or size of plankton to be observed
2. The Function
1.Ring: located above and serves as a binder and at owing rope plankton net. The ring is usually made of iron. Ring diameteris different- different brands and types depend on plankton net, but ingeneral,the diameter of this ring is 15 -25 cm.
2.String: serves to connect the net with a ring. The length of the rope varies depending on the type and the type of plankton net to be taken, but usually used rope measuring 25-50 cm
3.Wire: used to form the mouth of the net or net ting suit our wants and needs. Diameter of the wire is usually 31 cm to 45 cm for phyto plankton and zooplankton.
4.Nets: used usually of nylon material. Mesh size of the nets is typically 30-50 μm to 150-175 μm for phyto plankton and zooplankton, net length about 4-5 times the diameter of the mouth of the net.
5.Bottle / bucket: serves to store water sample has been filtered by the plankton net.
3. How to use it
Using a plankton net is easy. The trick is to drag the net through the water slowly. If you drag the net too fast, the water entering the net will not have time to exit through the mesh - no water going out means no more water coming in. No more water coming in means no more plankton.
There are four ways to drag the net through the water. The first is to tie the net to the stern of a boat and drag the net behind. The trick here is to move the boat slow enough so the water enters the net. The second method is to tie a weight to the net and lower the net deep into the water. Then slowly draw the net up through the water column. This technique works well if you are collecting plankton from a bridge that spans deep water. The third method involves tying the net to a long rope. While standing on the shore or a dock, swing the net back and forth, then let go, pitching the net out into the water. Once it lands, slowly draw the net back to shore. The fourth method is to attach the net to a long pole. The extendable poles that window washers use are ideal. These can be purchased at home improvement stores. Hold the end of the pol
4. How to care it
The holes should be spaced equidistant from each other with two sets of holes opposite each other.
Whether the weakness of the plankton net? and from the side where the difficulty will be experienced when using a plankton net? And the answer is  because the main ingredient is the manufacture of nylon plankton net, then the weakness is just not chlorine resistant. There is no difficulty in using plankton nets during follow instructions, and use of the plankton net is very simple.
16. OXYMETER
1. Description
Pulse oximetry is a non-invasive method allowing the monitoring of the saturation of a patient's hemoglobin.A sensor is placed on a thin part of the patient's body, usually a fingertip or earlobe, or in the case of an infant, across a foot. Light of two different wavelengths is passed through the patient to a photodetector. The changing absorbance at each of the wavelengths is measured, allowing determination of the absorbances due to the pulsing arterial bloodalone, excluding venous blood, skin, bone, muscle, fat, and (in most cases) nail polish.With NIRS it is possible to measure both oxygenated and deoxygenated hemoglobin on a peripheral scale (possible on both brain and muscle).

2. The Function
a. Pulse OxymeterDesktop,Monitor screen larger, more feature complete and can usually be connected to PC monitor screen. There is also an integrated Oxymeter with other medical monitoring devices, such as special operations Oxymeter in space.
b. Hand-held Pulse Oxymeter, Practical Oxymeter handful sized hands. Oxymeter is usually used for homecare patients are still frequently check-ups to the hospital.
3. How to use it
a. Main Unit, which consists of the Menu Manager (settings menu), such as: Set Alarm, Time, Setting Maximum and Minimum Levels of SpO2 and HR, etc.
b. Set Alarm, to set the maximum size of a high or low amount of oxygen required in the patient's body. If the oxygen level is higher or lower than the levels of SpO2 and HR has been determined, then the automatic alarm. Setting alarm by medical experts or nurses with a certain level limit, according to the needs of patients and cases.
c. LED Monitor: screen display for DTA-data value (amount) of oxygen within the patient's body such as the heart rate data, such as graphs or numbers. For this type of desktop Oxymeter usually LED Monitor can be connected using or PC computer.
d. Finger Sensor: special clamps to be mounted on the patient's finger. Equipment specially shaped "clamp" equipped infra-red to a very sensitive sensor to measure oxygen levels in the patient's body. Between 'clamp finger' with oximeter device connected by cable a monitor


Basicly the step of oxymeter is a sensor is placed on a thin part of the patient's body, usually a fingertip or earlobe, or in the case of an infant, across a foot.
4. How to care it
a.  After use, place Oxymeter on a clean place or in the cupboard.
b.  If Oxymeter dirty, immediately wipe using a cloth or tissue
c.  Do not drop or slam, because it can causeOxymeter damaged.
Impact the use oxymeters is all of the halth instrument always have positive effect and negative effect. we know in medicine if we drink over the medicine then has big effect. In here, the positive effect is can increase the haemoglobin in red blood cell, Very easy to use, portable, and what steps you need faster. In another version, you can even measure the calories burned and heart rate.
17.  AUXANOMETER
1. Description
Auxanometer ( Auxesis = growth; Meter = unit of length) of an instrument to measure the longitudinal growth of a plant, which consists of a pulley system fitted on the bow scale needle or needles which can scratch the cylinder.



2. The Function
Auxanometer is a tool to measure the longitudinal growth of a plant, which consists of the control system are equipped the needle on the scale arc or needles which can scratch the cylinder player.
3. How to use it
How to use auksanometer is as follows.
1) Tie a piece of string or thread at the end of the stem of the plant in a pot that had been prepared. Thread is placed on the pulley is placed just above the crop.
2) Then put the pulley pointing device which can rotate following the rotation of the pulley.
3) At the other end of the string attached to a ballast burden.
4) Set the pointer to the string pulleys to move along the arc before that had given scale.
5) Look for a few days arc accretion pointer and count how high or long rod that. 
But how can we determine the longitudinal growth of plant by using auxanometer? to determine the longitudinal growth of plant by using auxanometer you just look or observe the needle scale that have pointed.
4. How to care it
• Place auxanometer at room temperature chamber
• Do periodic cleaning
If you have disassembel before keep it and you have take it in the cupboard or another place that unreachable from dust, you just need clean it once in month or once in two month. but if you not disassembel and not keep it in the cupboard you should clean it every week
• Perform checks before and after using the tool
• Keep out of reach of children
• Do not clean the auxanometer are made of iron with water. In order to not get rusty auxanometer
• Disassemble auxanometer if not used in a long time, then keep it in the room temperature
Final task is to create a journal or a diary about laboratory techniques. A story of how early and what are the ups and downs in this course. Here I also tried to make the appearance like a diary so that interesting to read. And of course working with as much as possible.
I always instill in me is still trying as much as possible even though it is difficult to pass. Because here is part of my decision to take and certainly worth fighting for and I am accountable for my future. For parents, family and all the friends who have supported me, I can stay here and from here I will make them proud. For that, I still think if I start to complain. Because of the above I still have the better, and therefore I have to keep trying. And below I still have a shortage, therefore I have to stay humble and willing to help others. When the rigors of life began to feel I could say to myself,
"Life is an adventure. And certainly there are ups and downs in it. So enjoy it all " J
Thank you Mrs. Erlia Narulita, S.Pd., M.Si and Mrs. Kamalia Fikri, S. Pd., M. Pd which have guided the course of this laboratory techniques. Without the guidance may not easily through it. May all that has been learned can be useful to me, other people, religion and country, especially for the development of laboratory techniques that have been there. J    


                                   Signed
                                    Bella Rhea Lavifa Sanjaya




 















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