Tugas Teknik Laboratorium

MICROTOME

A microtome (from the Greek mikros, meaning "small", and temnein, meaning "to cut") is a tool used to cut extremely thin slices of material, known as sections. Important in science, microtomes are used in microscopy, allowing for the preparation of samples for observation under transmitted light orelectron radiation. Microtomes use steel, glass, or diamond blades depending upon the specimen being sliced and the desired thickness of the sections being cut. Steel blades are used to prepare sections of animal or plant tissues for light microscopy histology. Glass knives are used to slice sections for light microscopy and to slice very thin sections for electron microscopy. Industrial grade diamond knives are used to slice hard materials such as bone, teeth and plant matter for both light microscopy and for electron microscopy. Gem quality diamond knives are used for slicing thin sections for electron microscopy.

The Function of Microtome

Microtomy is a method for the preparation of thin sections for materials such as bones, minerals and teeth, and an alternative to electropolishing and ion milling. Microtome sections can be made thin enough to section a human hair across its breadth, with section thickness between 50 nm and 100 µm.

All microtomes consist of three main parts:

·         Base (microtome body)

·         Knife attachment and knife

·         Material or tissue holder

Treatment Microtome

Microtome should be covered with plastic, or put back in the box when not in use. Do not move microtome by holding the moving parts, as they may interfere with the accuracy. Before and after use, preferably paraffin microtome cleared of debris by wiping with a cloth that has been dampened with xilol. Microtome must be oiled to prevent wear and congestion.

To buy the microtome, we can buy it onlinely on website

http://microtomedepot.com/product.html

Or contact them toll free 1-877-215-3795

And via email : sales@microtomedepot.com

Hand & Table Microtome

Model: MICROTOME-1: RMT-5

Price: $ 90.00

Shipping and Handling: $ 18.00

It is a portable, handy and simple Microtome recommended for laboratories, field work etc. for sectioning of fresh tissues. It is easy to operate and cuts in steps of 10 microns each.

All components are fabricated from standard metals, Top stage is of high ground GLASS for smooth operations.

Supplied complete in a wooden box with a plano concave razor, block holder, mould block, table clamping device and operating instructions.

microtome

Rocking Microtome (Cambridge Type)

Model: MICROTOME-2: RMT-10

Price: $ 280.00

Shipping and Handling: $ 25.00

This is a sturdy and well designed microtome (Cambridge type) which can cut sections of tissues from 2-24 microns in steps of 2 microns each. It is highly suitable for elementary students.

Supplied with razor with built-in handle in box, 3 block holders, Dust cover & honing stone in a storing cabinet.

            

Rotary Microtome (Erma Type)

Model: MICROTOME-3: RMT-20

Price: $ 470.00

Shipping and Handling: $ 50.00

Standard & economically priced sectioning device providing all advantages of precision Rotary Microtome. The interior mechanism is seated on a heavy cast iron base, covered with a metal cover for protection from dust and grit. The Microtome is equipped with a ball and flange type object holder to get exact centering of specimen, knife holder with lateral movement permitting use of complete knife edge and has feed range from 1-25 microns in steps of 1 micron each.

Senior Precision Rotary Microtome
Model: MICROTOME-4: RMT-30
Price: $ 675.00
Shipping and Handling: $ 55.00

It is a universally approved, most modern and reliable sectioning equipment designed for very precise sectioning of tissues upto 1 micron thickness. The feed indicator, located in front of microtome is operated by the latest 'CAM DRIVE SYSTEM' for accurate feed setting from 1 - 50 micron in steps of 1 micron.

The salient features include independent feed mechanism with automatic safety device, universal knife holder with lateral movements permitting use of the entire knife edge, universal vice type object holder for accurate centering of the specimen.

Freezing Microtome
Model: MICROTOME-5: RMT-40
Price: $ 410.00
Shipping and Handling: $ 30.00

Model RMT-40 is a very compact and sturdy model of Freezing Microtome having a table clamping device for fixing the instrument to the side of any standard table. It has a feed range from 5-30 microns in step of 5 microns each. Its freezing attachment has a specimen stage of 15mm dia. and a high pressure flexible tubing approx 150cms. for connecting to the Carbon Diaoxide Cylinder. The Microtome razor is a high quality steel knife tested for micro-structures and specially designed for cutting frozen specimens. Supplied with Illustrated Operating Instructions & following accessories:

Razor 100mm with back & handle - 1 No.
Honing Stone - 1 No.
Freezing Attachment - 1 No.
Oil Can - 1 No.
Dust Cover - 1 No.
Wooden Cabinet - 1 No.